Mapping bovine herpesvirus type 1 latency-related RNA in trigeminal ganglia of latently infected rabbits
نویسندگان
چکیده
منابع مشابه
Detection of bovine herpesvirus type 1 RNA in trigeminal ganglia of latently infected rabbits by in situ hybridization.
At times after conjunctival inoculation with bovine herpesvirus type 1 (BHV-1), representing the acute and latent phases of infection, rabbit trigeminal ganglia were examined for the presence of BHV-1 nucleic acids by in situ hybridization using a 3H-labelled BHV-1 DNA probe. During the acute phase of virus infection, both BHV-1 DNA and RNA were detected in ganglionic neurons and occasionally i...
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Bovine herpesvirus 1 (BoHV-1) is one of the most important infectious viruses of cattle which is considered as the causative agent of several clinical conditions in this species. Hence, we tried to isolate a local strain of the virus from carrier cattle in Ahvaz based on a strategy for reactivation of the latent infection in hosts having antibodies against the virus. Thus, 20 cattle were examin...
متن کاملLatency-associated transcripts of equine herpesvirus type 4 in trigeminal ganglia of naturally infected horses.
Equine herpesvirus type 4 (EHV-4) is a major respiratory pathogen of horses. Unlike most other members of the Alphaherpesvirinae, EHV-4 was regarded as non-neurotropic. Here, neural and lymphoid tissues of 17 horses have been analysed post-mortem. EHV-4 DNA was detected in 11 cases (65%) by PCR, exclusively in the trigeminal ganglia. In order to define the transcriptional activity, RNA preparat...
متن کاملAnalysis of cyclins in trigeminal ganglia of calves infected with bovine herpesvirus-1.
Following acute infection of cattle with bovine herpesvirus-1 (BHV-1), cyclin expression was examined in trigeminal ganglia (TG). Cyclin A was primarily detected in the nucleus of TG neurons. In contrast, cyclin D1 and cyclin E were detected in the nucleus and cytoplasm of TG neurons. Uninfected or latently infected calves did not express detectable levels of these cyclins in TG neurons. Follow...
متن کاملIdentification of a novel bovine herpesvirus 1 transcript containing a small open reading frame that is expressed in trigeminal ganglia of latently infected cattle.
Bovine herpesvirus 1 (BHV-1), like other Alphaherpesvirinae subfamily members, establishes latency in sensory neurons. The latency-related (LR) RNA is abundantly expressed during latency, and expression of an LR protein is required for the latency reactivation cycle in cattle. Within LR promoter sequences, a 135-amino-acid open reading frame (ORF) was identified, ORF-E, that is antisense to the...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1987
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.61.12.3827-3831.1987